Molecular Biology Insights Oligo v7.52 引物設計軟體 英文破解版   軟體簡介: OLIGO是一個從序列檔中搜索和選擇寡核酸的多功能程式,應用于聚合鏈反應(PCR), DNA測序,定點突變及各種各樣的雜交研究。 OLIGO根據最近鄰熱力學值計算寡核酸的雜交溫度和二級結構。這個好工具也用於構 建合成基因,在已合成的基因中發現適當的序列引物,發現和多元化一致的引物和探 針,甚至發現蛋白質潛在的限制位點。 OlIGO產品特色: 1.分析TM和內部穩定性,繪製溶解溫度圖和穩定性圖; 2.給出寡核?酸的重要相關資訊; 3.顯示正向引物、反向引物和當前的OLIGO裏潛在的連接物。潛在的二聚物也可顯示出來; 4.分析顯示在選中的正向引物或反向引物中基本的成份和溶解溫度; 5.精確地分析出生物資訊軟體中的錯配位元點; 6.正向引物和反向引物選中後,能自動產生PCR實驗條件和PCR產物資訊。 OLIGOPrimerAnalysisSoftwareistheessentialtoolfordesigningand analyzingsequencingandPCRprimers,syntheticgenes,andvariouskindsof probesincludingsiRNAandmolecularbeacons. Basedonthemostup-todatenearestneighborthermodynamicdata,Oligo's searchalgorithmsfindoptimalprimersforPCR,includingTaqMan,highly multiplexed,consensusordegenerateprimers.Multiplefilebatch processingispossible.Itisalsoaninvaluabletoolforsitedirected mutagenesis. Foreachprimerorprimerpair,Oligo'svariousanalysiswindowsshowa multitudeofusefuldata,suchasDNAandRNAsecondarystructure,dimer formation,falseprimingandhomology,internalstability,compositionand physicalproperties.WithOligoyoucananalyzeopenreadingframesdownto predictedmolecularweightandpKaofproteins,andsearchforrestriction enzymesites,notonlyinDNAbutalsoinreverse-translatedproteins. Thefirstversionofthesoftwareappearedonthemarketin1989.Itwent throughseveralmodifications,andthelastone,thechangefromversion6 to7,wasthemostcomprehensive.Oligo7canautomaticallyselectmultiplex primers,processsequencefilesinbatchmodes,automaticallydesignPCR primerstocovermultipleDNAregionsinjustonesearchandautomatically findprimer/probesetsforrealtimePCRorfindsnestedprimerssets.Oligo searchprotocols(scoringsystem)maybecustomizedindetail,soyoumay optimizetheresultsaccordingtoyourspecificneeds.